| Notes | |
|---|---|
| Storage Buffer | |
| Similar Names | |
| Clone Number | |
| Isotype | |
| Positive Control | |
| Chemical Name | RAW 264.7 |
| Remark | RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤；sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒，XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖，并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球，但对肿瘤靶细胞无作用。 |
| Storage Instructions | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| Cell Name | RAW 264.7 |
| Growth Medium | DMEM＋10% FBS＋1% P/S |
|---|---|
| Species Reactivity | |
| Growth | 贴壁细胞，不用胰酶消化 |
| Antigen Exp | H-2d |
| Applications | |
| Conjugation | |
| Clone | |
| Storage Condition | |
| Gene Exp | lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox). |
| Doubling Time | ~12-30 hours |
| Receptor Exp | complement (C3) |
| Immunogen | |
| Host Species | |
| Generation Ratio | 1:6-1:8 |
| Characters | 不规则形 |
| Culture Conditions | 气相：空气，95%；CO2，5%, 温度：37℃ |
|---|---|
| BSL | BSL-2 |
| Notes | 1.该细胞不建议使用胰酶消化，胰酶会刺激分化；2.超过3天不传代细胞容易分化；3.培养时，存在少量分化细胞，属于正常现象。 |
| Liquid Frequency | 2-3次/周 |
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