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| Chemical Name | HK-2 |
| Remark | HK-2细胞属源于正常肾的近曲小管细胞，通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2；Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317，最后将PA317细胞产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性，但未用G418筛选转导克隆。Southern和FISH分析显示，HK-2细胞来源于单克隆。PCR检测证实，HK-2细胞基因组中含有E6/E7基因。 |
| Storage Instructions | 气相：空气，95%；CO2，5%, 温度：37℃ |
| Cell Name | HK-2 |
| Growth Medium | MEM（含NEAA）＋10% FBS＋1% P/S |
|---|---|
| Species Reactivity | |
| Growth | 贴壁细胞 |
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| Host Species | |
| Generation Ratio | 1:2-1:4 |
| Characters | 上皮细胞样 |
| Culture Conditions | |
|---|---|
| BSL | BSL-2 |
| Notes | |
| Liquid Frequency | 2-3次/周 |
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